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Production of xylooligosaccharides by xylanase from Pichia stipitis based on xylan preparation from triploid Populas tomentosa.

Identifieur interne : 002D48 ( Main/Exploration ); précédent : 002D47; suivant : 002D49

Production of xylooligosaccharides by xylanase from Pichia stipitis based on xylan preparation from triploid Populas tomentosa.

Auteurs : Haiyan Yang [République populaire de Chine] ; Kun Wang ; Xianliang Song ; Feng Xu

Source :

RBID : pubmed:21565493

Descripteurs français

English descriptors

Abstract

Xylooligosaccharides (XOS) with DP 2-4 are important synbiotics used as food ingredients based on its prebiotic characteristics. In this work, the production of XOS from lignocellulosic material was performed by combined chemical-enzymatic methods. Xylan was prepared from triploid Populas tomentosa, and bioconverted into XOS by crude xylanase solution obtained from Pichia stipitis. The effects of reaction time, temperature, enzyme dosage, and pH value on the production of XOS were fully evaluated. Under the optimal condition (25U g(-1) substrate, pH 5.4 and 50°C), 36.8% of the xylan preparation was converted to XOS, equivalent to 3.95 mg/mL of the hydrolyzate. Xylobiose, xylotriose and xylotetrose were analyzed to be the main products of the enzymatic hydrolyzate, which together accounted for over 95% of the released oligosaccharides. Meanwhile, the effect of sonication pretreatment on the conversion efficiency of the xylan preparation was also investigated.

DOI: 10.1016/j.biortech.2011.03.110
PubMed: 21565493


Affiliations:


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Le document en format XML

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<name sortKey="Song, Xianliang" sort="Song, Xianliang" uniqKey="Song X" first="Xianliang" last="Song">Xianliang Song</name>
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<name sortKey="Xu, Feng" sort="Xu, Feng" uniqKey="Xu F" first="Feng" last="Xu">Feng Xu</name>
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<term>Hydrogen-Ion Concentration (MeSH)</term>
<term>Hydrolysis (MeSH)</term>
<term>Molecular Weight (MeSH)</term>
<term>Oligosaccharides (biosynthesis)</term>
<term>Pichia (enzymology)</term>
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<term>Temperature (MeSH)</term>
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<term>Concentration en ions d'hydrogène (MeSH)</term>
<term>Endo-1,4-beta xylanases (métabolisme)</term>
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<term>Masse moléculaire (MeSH)</term>
<term>Oligosaccharides (biosynthèse)</term>
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<term>Pichia (enzymologie)</term>
<term>Populus (composition chimique)</term>
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<front>
<div type="abstract" xml:lang="en">Xylooligosaccharides (XOS) with DP 2-4 are important synbiotics used as food ingredients based on its prebiotic characteristics. In this work, the production of XOS from lignocellulosic material was performed by combined chemical-enzymatic methods. Xylan was prepared from triploid Populas tomentosa, and bioconverted into XOS by crude xylanase solution obtained from Pichia stipitis. The effects of reaction time, temperature, enzyme dosage, and pH value on the production of XOS were fully evaluated. Under the optimal condition (25U g(-1) substrate, pH 5.4 and 50°C), 36.8% of the xylan preparation was converted to XOS, equivalent to 3.95 mg/mL of the hydrolyzate. Xylobiose, xylotriose and xylotetrose were analyzed to be the main products of the enzymatic hydrolyzate, which together accounted for over 95% of the released oligosaccharides. Meanwhile, the effect of sonication pretreatment on the conversion efficiency of the xylan preparation was also investigated.</div>
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<CopyrightInformation>Copyright © 2011 Elsevier Ltd. All rights reserved.</CopyrightInformation>
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